Tubulin Delivery: Polymerization Chaperones for Microtubule Assembly?
نویسندگان
چکیده
منابع مشابه
Heterogeneous Tau-Tubulin Complexes Accelerate Microtubule Polymerization.
Tau is an intrinsically disordered protein with a central role in the pathology of a number of neurodegenerative diseases. Tau normally functions to stabilize neuronal microtubules, although the mechanism underlying this function is not well understood. Of note is that the interaction between tau and soluble tubulin, which has implications both in understanding tau function as well as its role ...
متن کاملPolymerization of tubulin in vivo: direct evidence for assembly onto microtubule ends and from centrosomes
Microtubule assembly in vivo was studied by hapten-mediated immunocytochemistry. Tubulin was derivatized with dichlorotriazinylaminofluorescein (DTAF) and microinjected into living, interphase mammalian cells. Sites of incorporation were determined at the level of individual microtubules by double-label immunofluorescence. The haptenized tubulin was localized by an anti-fluorescein antibody and...
متن کاملThe nucleotide switch of tubulin and microtubule assembly: a polymerization-driven structural change.
GTP-binding proteins from the tubulin family, including alphabeta-tubulin, gamma-tubulin, bacterial tubulin, and FtsZ, are key components of the cytoskeleton and play central roles in chromosome segregation and cell division. The nucleotide switch of alphabeta-tubulin is triggered by GTP hydrolysis and regulates microtubule assembly dynamics. The structural mechanism of the switch and how it mo...
متن کاملBrain tubulin polymerization in the absence of "microtubule-associated proteins".
Pure tubulin dimer, purified by phosphecellulose chromatography to remove microtubule associated proteins, can be assembled into microtubules in the presence of dimethyl sulfoxide. The surfaces of such microtubules are devoid of filamentous material. The reaction is rapid and strongly dependent on protein concentration. No microtubule formation occurs from the purified tubulin when dimethyl sul...
متن کاملHypothiocyanous acid oxidation of tubulin cysteines inhibits microtubule polymerization.
Thiol oxidation is a probable outcome of cellular oxidative stress and is linked to degenerative disease progression. In addition, protein thiol redox reactions are increasingly identified as a mechanism to regulate protein structure and function. We assessed the effect of hypothiocyanous acid on the cytoskeletal protein tubulin. Total cysteine oxidation by hypothiocyanous and hypochlorous acid...
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ژورنال
عنوان ژورنال: Developmental Cell
سال: 2007
ISSN: 1534-5807
DOI: 10.1016/j.devcel.2007.09.009